Article
Extended Data Table 3 | Details of the synthetic HCoV-HKU1, SARS-CoV-2, MERS-CoV Riyadh-1734-2015 and ZIKA virus
fragments
Fragment Fragment size(bp) Cloning vector Antibioticselection^Restriction sites5'-end 3'-end 5'-end 3'-end (^) digestionVector^
HCoV-HKU1
Fragment 1 1 2399 2506 pUC5 7 AmpR (^)
Fragment 2 8526 10920 2395 pUC5 7 AmpR (^)
Fragment 3 19107 21530 2424 pUC5 7 AmpR (^)
Fragment 4 27484 29925 2646 pUC5 7 AmpR (^)
SARS-CoV-2
Fragment 1. 1 1 483 579 pUC5 7 AmpR SmaI SmaI
Fragment 1. 2 1 483 579 pUC5 7 AmpR SmaI SmaI
Fragment 1. 3 1 483 579 pUC5 7 AmpR SmaI SmaI
Fragment 2 377 3325 2949 pUC5 7 AmpR SmaI SmaI PvuI
Fragment 3 3012 6315 3304 pUC5 7 AmpR EcoRV EcoRV PvuI
Fragment 4 6003 8994 2992 pUC57mini AmpR EcoRV NdeI
Fragment 5 8718 11966 3249 pCC1-His3 ChloR SmaI SmaI
Fragment 5A 8718 10475 1757 pUC1 9 AmpR SmaI
Fragment 5B 10446 11023 573 pUC1 9 AmpR (^)
Fragment 5C 10994 11540 573 pUC1 9 AmpR (^)
Fragment 5D 11511 11966 456 pUC1 9 AmpR SmaI
Fragment 6 11664 14605 2942 pUC57mini AmpR SmaI SmaI
Fragment 7 14311 17698 3388 pCC1-His3 ChloR SmaI SmaI
Fragment 7Aa 14311 15445 1134 SmaI
Fragment 7Ab (^15416 16029 614)
Fragment 7B 16000 17698 1699 pUC5 7 AmpR SmaI
Fragment 8 17399 20358 2960 pUC57mini AmpR SmaI SmaI
Fragment 9 20110 23286 3177 pUC57mini AmpR SmaI SmaI
Fragment 10 22975 25940 2966 pUC57mini AmpR SmaI SmaI
Fragment 11 25595 28779 3185 pUC57mini AmpR SmaI SmaI
Fragment 12 28274 29870 1812 pUC5 7 AmpR SmaI SmaI
pCC1-MERS-CoV-Riyadh-1734-2015
Fragment (^1 1 2190 2339)
Fragment (^8 28447 30126 1791) pUC5 7 Amp
ZIKA virus
Fragment 6 10627 10807 298 pUC5 7 Amp
R
R
Genome position
(5’ UTR to 3’ UTR)