Nature - USA (2020-06-25)

Article

OH

O

O

H

H

H

H

OH

SCoA

O

O

H

H

H

H

OH

SCoA

O

HO H

H

H

H

H

OH

OH

SCoA

O

H

H

H

H

OH

O OH

OH

O

HO H

H

H

H

H

OH

OH

OH

O

O

H

H

H

H

OH

OH

O

O H

H

H

H

H

OH

OH

O

HO H

H

H

H

H

OH

3-oxo-DCADCA

3-oxo-4,5-6,7-

didehydro-DCA

3-oxo-4,5-

dehydro-DCA

CA

??

?

H

SCoA

O

O

H

H

H

H

OH

OH

BaiB BaiA2

BaiCD

BaiF BaiE

3-oxo-

cholyl-CoA

cholyl-CoA

3-oxo-4,5-

dehydro-

cholyl-CoA

3-oxo-4,5-6,7-

didehydro-deoxy-

cholyl-CoA

Extended Data Fig. 1 | Previously proposed pathway for 7α-dehydroxylation of cholic acid in C. scindens VPI 12708. See main text for details and a summary of
the previous literature. CA, cholic acid.