Nature - USA (2020-06-25)

Article

a

c

Cell loading port Outlet

Loading port Outlet

Confinement channelsMicro- Confinement

b

f

2.5D

Serrated channel

Smooth channel

Cell speed (μm/min)

pNB+EDTA

3D view

side view

20 40 60 80 100 120

0

10

20

30

40

50

+EDTA (10mM)

2.5D

Serrated channel

Smooth channel

(^020406080100120)
20
40
50
30
10
+EDTA (10mM)
+paraNitroBlebbistatin (50μM)
Cell speed treatment (%beforeafter )
0
100
200
300

---

ns
0
5
10
15
20

• 
  
  
  
  • 
    
  
  
  
  

2.5D

****

0

5

10

15

20

• 
  
  
  
  • 
    
  
  
  
  

Serrated

channel

****

0

5

10

15

20

• 
  
  
  
  • 
    
  
  
  
  

Smooth

channel

****

Cell speed (μm/min)

pNB

0

5

10

15

20 ****

• 
  
  
  
  • 
    
  
  
  
  

Smooth

channel

0

5

10

15

20 ****

• 
  
  
  
  • 
    
  
  
  
  

Serrated

channel

0

5

10

15

20 ****

• 
  
  
  
  • 
    
  
  
  
  

2.5D

3D view

side view

0

100

200

300

ns ns

ns

+paraNitroBlebbistatin (50μM)

Cell frequency (%

)

Cell speed pNB+EDTA (%

)

beforeafter

2.5D

Serrated channel

Smooth channel

Cell speed pNB (%

)

beforeafter

d e

0

10

20

30

40

50

20 40 60 80 100 120

Cell speed treatment (%beforeafter ) Cell speed treatment (%beforeafter )

g

0s15s30s45s60s

t=0min t=8min t=20mint=70min

60s

0s

Lifeact-mCherry

Brigthfield

Cell

Actin

flow

0

5

10

15

20

25

EDTA

6μm 12 μm 24μmSmooth

Velocity (μm/min

)

Channel

zone

*

ns

***

Extended Data Fig. 5 | See next page for caption.