Nature - USA (2020-01-02)

Antibody titers (BAL wash)

log

10

(endpoint titer)

Weeks after BCG immunization

Antibody titers (plasma)

Weeks after BCG immunization

a b

0

2

4

0

2

4

6

P 2 4 8 12 16 20 24

IgG

IgA

0

2

4

6

0

2

4

6

P 4 12 24

IgG

IgA

2

4

IgM

4

(^6) IgM
0
2
0
AE
AE/ID
IV
IDlow
IDhigh
BCG Regimen
<0.001
0.014
0.008
<0.001
<0.001
0.020
0.005
<0.001
<0.001
<0.001
<0.001
0.412

0.999
log
10
(endpoint titer)
log
10
(midpoint titer)
Extended Data Fig. 7 | Humoral immune response in BAL and plasma after
BCG immunization. Mtb-responsive antibody responses were assessed in BAL
and plasma after BCG immunization. Mtb WCL-specific IgG, IgA and IgM
antibody titres were measured from individual NHPs at various time points
before and after BCG immunization. Shown are end-point titres for IgG and IgA
and mid-point titres for IgM (in which the end point was not reached) a,
Antibody titres in tenfold-concentrated BAL f luid (cohorts 1–4, n = 1 1–1 3
macaques except at weeks 2, 20 and 24, cohort 4 only, n = 3 macaques). In
concentrated BAL f luid, antigen-responsive IgG, IgA and IgM were detected
only in IV-BCG-immunized NHPs and returned to pre-vaccination levels by the
time of challenge. b, Antibody titres in plasma (n = 11–13 macaques). In plasma,
both IDhigh and IV BCG elicited increased IgG and IgA antibody responses
compared to IDlow BCG. Data are geometric mean and s.d.; dashed line indicates
assay limit of detection. A Kruskal–Wallis test was used to compare all vaccine
groups to IDlow at weeks 4, 16 and 24 (BAL) or weeks 4 and 24 (plasma); P values
are from Dunn’s multiple comparison test (colour-coded to vaccine).