Nature - USA (2020-01-02)

IFN

ivCD45

0

1

2

3

4

5

PBMC

Bone marrow

Spleen

Liver

Skin

Axillary LNInguinal LNMesenteric LN

Hilar LNCarinal LN

BALRU

LR LRLR LR

RMRLLULMAcc

Lung

lobes

0

1

2

3

4

5

PBMC

Bone marrow

Spleen

Liver

Axillary LNInguinal LNMesenteric LN

Hilar LNCarinal LN

BALRU

LRLR LR

RMRLLULMAcc

Lung

lobes

0

1

2

3

4

5

PBMC

Bone marrow

Spleen

Liver

Axillary LNInguinal LNMesenteric LN

Hilar LN

BALRU

LRLR R

RMRLLULMAcc

Lung

lobes

0

1

2

3

4

5

PBMC

Bone marrow

Spleen

Liver

Axillary LNInguinal LNMesenteric LN

Hilar LNCarinal LN

BALRU

LRLR LR

RMRLLULMAcc

Lung

lobes

Pre-infusion Systemic

PBMCPost-infusionPBMC B. marrow Spleen

L. axillary R. axillary L. inguinal R. inguinal Mesenteric

L. hilar R. hilar Carinal

BAL R. upper R. middle R. lowerL. upperL. lower Accessory

Peripheral

LN

LNLung

BAL &

lung lobes

IDhigh

log

(BCG CFU) 10

IV

AE

EB

a

Pre-infusion Systemic

PBMCPost-infusionPBMC B. marrow Spleen

L. axillary R. axillary L. inguinal R. inguinalMesenteric

L. hilar R. hilar Carinal

BAL R. upper R. middle R. lowerL. upper L. lower

Peripheral

LN

BAL &

lung lobes

Pre-infusion Systemic

PBMC

Post-infusion

PBMC

B. marrow Spleen

L. axillary R. axillary L. inguinal R. inguinal Mesenteric

L. hilar

BAL R. upper R. middle R. lowerL. upperL. lower

Peripheral

LN

Accessory BA

L &

lung lobes

Pre-infusion Systemic

PBMC

Post-infusion

PBMC

B. marrow Spleen

L. axillary R. axillary L. inguinal R. inguinalMesenteric

L. hilar R. hilar Carinal

BAL R. upper R. middle R. lowerL. upper L. lower

Peripheral

LN

Accessory lung lobesBAL &

LNLung

LNLung

IFN

IFN

N

FN

FN

b

c

d

log

(BCG CFU) 10

log

(BCG CFU) 10

log

(BCG CFU) 10

Extended Data Fig. 12 | See next page for caption.