Article
Extended Data Table 3 | Conservation of RIPK1 caspase-8 cleavage site
Protein sequences orthologous to human RIPK1 were aligned in 235 vertebrate species, using Multiz alignment in the UCSC Genome Browser. These include representative species from the
major classes: 51 fish, 3 amphibians (A.), 14 reptiles (Rept.), 58 birds (Aves) and 109 mammals (Mammalia). Most species within these classes, except fish (7 out of 51), contain the very highly
conserved D324 (human numbering) caspase cleavage site within this region. Notably, nearly all species (223) have a potential caspase cleavage site, D300; however, it is noteworthy that this
Asp is in most cases succeeded by a large hydrophobic amino acid that is less favourable for caspase cleavage.