3
nature research | reporting summary
October 2018- H3K79me2: Manufacturer indicates human and mouse reactivity by immunoplot. We have demonstrated in prior work (Wan
et al. 2017) that this antibody can be used for ChIP-seq. - E-cadherin: Manufacturer indicates human and mouse reactivity by IF.
- WT-1: Manufacturer indicates human and mouse reactivity by IF.
- Biotinylated Lotus Tetragonolobus Lectin (LTL): Manufacturer indicates application for IF staining.
Eukaryotic cell lines
Policy information about cell lines
Cell line source(s) HEK293, HK-2 lines purchased from ATCC; mESCs cells derived in houseAuthentication For cells purchased directly from ATCC, cells are authenticated by sequencing at ATCC. During culture, parental lines were
authenticated based on the testing and monitoring of phenotypic features (morphology, differentiation potential, growth
conditions, etc.) characteristic of each line that were previously reported by manufacturers and other groups. Immunoblot
analysis was performed to confirm the genotypes after transgene/siRNA expression.Mycoplasma contamination All cell lines tested negative for mycoplasma contamination.Commonly misidentified lines
(See ICLAC register)No commonly misidentified lines were used in this study.Animals and other organisms
Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research
Laboratory animals For^ laboratory^ animals,^ report^ species,^ strain,^ sex^ and^ age^ OR^ state^ that^ the^ study^ did^ not^ involve^ laboratory^ animals.Wild animals Provide^ details^ on^ animals^ observed^ in^ or^ captured^ in^ the^ field;^ report^ species,^ sex^ and^ age^ where^ possible.^ Describe^ how^ animals^
were caught and transported and what happened to captive animals after the study (if killed, explain why and describe method; if
released, say where and when) OR state that the study did not involve wild animals.Field-collected samples For laboratory work with field-collected samples, describe all relevant parameters such as housing, maintenance, temperature,
photoperiod and end-of-experiment protocol OR state that the study did not involve samples collected from the field.Ethics oversight Identify^ the^ organization(s)^ that^ approved^ or^ provided^ guidance^ on^ the^ study^ protocol,^ OR^ state^ that^ no^ ethical^ approval^ or^
guidance was required and explain why not.Note that full information on the approval of the study protocol must also be provided in the manuscript.
ChIP-seq
Data deposition
Confirm that both raw and final processed data have been deposited in a public database such as GEO.Confirm that you have deposited or provided access to graph files (e.g. BED files) for the called peaks.Data access links
May remain private before publication.https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE125186
Token: gdwbimyqlhyxlarFiles in database submission GSM3564782 ChIPSeq.PolII_S2P_T1_mut
GSM3564783 ChIPSeq.PolII_S2P_T2_mut
GSM3564784 ChIPSeq.PolII_S2P_T3_mut
GSM3564785 ChIPSeq.PolII_S2P_WT_ENL
GSM3564786 ChIPSeq.CDK9_T1_mut
GSM3564787 ChIPSeq.CDK9_T2_mut
GSM3564788 ChIPSeq.CDK9_T3_mut
GSM3564789 ChIPSeq.CDK9_WT_ENL
GSM3564790 ChIPSeq.HEK293_WT_ENL_B
GSM3564791 ChIPSeq.HEK293_T1_mut_C
GSM3564792 ChIPSeq.HEK293_T1_Y78A_mut
GSM3564793 ChIPSeq.HEK293_T2_B_mut
GSM3564794 ChIPSeq.HEK293_T2_Y78A_mut
GSM3564795 ChIPSeq.HEK293_T3_B_mut
GSM3564796 ChIPSeq.HEK293_T3_Y78A_mut
GSM3564797 ChIPSeq.HEK293_vec
GSM3564798 ChIPSeq.HEK293_Y78A_B_mut
GSM3564799 ChIPSeq.HEK293_F_ENL
GSM3564800 ChIPSeq.HEK293_F_T1_mut
GSM3564801 ChIPSeq.HEK293_F_T2_mut