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nature research | reporting summary
April 2018Animals and other organisms
Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research
Laboratory animals Caenorhabditis elegans hermaphrodites were the only experimental organism used in this study. RNAi screening was done at day
6 of adulthood. For aggregate counting, animals at day 2, day 4, day 6, day 8, day 12 of adulthood were used. Evaluation of
endocytosis was performed at day 1 of adulthood. For insoluble protein extractions, animals of day 2 and day 10 were used. Co-
purification was performed at day 3 of adulthood. For qRT-PCR, day 1 adults were used for all experiments except to test
expression levels with age, where animals at day 2 and day 8 of adulthood were used. RNAseq was performed with day 1 adults.
Strains used are the following:
DCD1 uqEx1[plbp-2::lbp-2::tagrfp]
DCD23 uqIs5[plbp-2::lbp-2::tagrfp]
CF512 fer-15(b26) II; fem-1(hc17) IV
DCD130 fer-15(b26) II; fem-1(hc17) IV; uqIs5[plbp-2::lbp-2::tagrfp]
DCD320 uqEx55[plys-7::lys-7::tagrfp; podr-1::cfp]
GS1912 dpy-20(e1282) IV; alrs37[pmyo-3::ssgfp; dpy-20(+)]
NP717 unc-119(ed3); arls37[pmyo-3::ssgfp; dpy-20(+)]; cdls32[pcc1::DT-A(E148D); unc-119(+); pmyo-2::gfp]
DCD378 arls37[pmyo-3::ssgfp; dpy-20(+)]
DCD379 unc-119(ed3); arls37[pmyo-3::ssgfp; dpy-20(+)]; cdls32[pcc1::DT-A(E148D); unc-119(+); pmyo-2::gfp]
DCD308 uqEx52[pmyo-3::clec-1::mVenus::histag]; uqIs5[plbp-2::lbp-2::tagrfp]
DCD311 uqEx53[pmyo-3::C36C5.5::mVenus::histag]; uqIs5[plbp-2::lbp-2::tagrfp]
DCD312 uqEx54[pmyo-3::F56B6.6::mVenus::histag]; uqIs5[plbp-2::lbp-2::tagrfp]
DCD363 uqIs27[pmyo-3::C36C5.5::mVenus::histag]; uqIs5[plbp-2::lbp-2::tagrfp]
DCD362 fer15(b26) II; fem-1(hc17) IV; uqIs27[pmyo-3::C36C5.5::mVenus::histag]; uqIs5[plbp-2::lbp-2::tagrfp]
DCD401 uqEx61[pmyo-3::lys-3::mVenus::histag]; uqIs5[plbp-2::lbp-2::tagrfp]
DCD381 unc-119(ed3); arls37[pmyo-3::ssgfp; dpy-20(+)]; cdls32[pcc1::DT-A(E148D); unc-119(+); pmyo-2::gfp];
uqIs5[plbp-2::lbp-2::tagrfp]
DCD383 uqIs5[plbp-2::lbp-2::tagrfp]; arls37[pmyo-3::ssgfp; dpy-20(+)]
DCD371 uqIs5[plbp-2::lbp-2::tagrfp]; arls37[pmyo-3::ssgfp; dpy-20(+)]
DCD387 unc-119(ed3); cdls32 [(pcc1::DT-A(E148D); unc-119(+); pmyo-2::gfp]; uqIs27[pmyo-3::C36C5.5::mVenus::hisTag];
uqIs5[plbp-2::lbp-2::tagrfp]
DCD403 uqEx62[plbp-2::tagrfp]
DCD398 tag-196(ok822) V; uqEx1[plbp-2::lbp-2::tagrfp]
DCD370 clec-1(tm1291) V; uqEx1[plbp-2::lbp-2::tagrfp]
DCD400 uqEx56[plys-7::lys-7::tagrfp; podr-1::cfp]; uqIs31[pmyo-3::clec-1::mVenus::histag]
DCD405 lys-3(tm2505) V; uqEx1[plbp-2::lbp-2::tagrfp]
DCD406 dod-21 & C32H11.1(ok1569) IV; uqEx1[plbp-2::lbp-2::tagrfp]
DCD359 jkk-1(km2) X; uqIs5[plbp-2::lbp-2::tagrfp]
DCD360 jnk-1(gk7) IV; uqIs5[plbp-2::lbp-2::tagrfp]
DCD361 kgb-1(km21) IV; uqIs5[plbp-2::lbp-2::tagrfp]
KU21 kgb-1(km21) IV
KU2 jkk-1(km2) X
VC8 jnk-1(gk7) IV
RB1384 dod-21 & C32H11.11(ok1569) IV
RB939 tag-196(ok822) V
FX02505 lys-3(tm2505) V
FX01291 clec-1(tm1291) V
DCD410 uqIs5[plbp-2::lbp-2::tagrfp]; uEx60 [punc-119::yfp; punc-119::sid-1]Wild animals The study did not involve wild animalsField-collected samples The study did not involve samples collected from the field