Article
Extended Data Fig. 1 | Growth curves for shifts. a, Growth curves following
shifts from different glycolytic carbons to acetate by filtration. Long lag phases
can consist of several hours without detectable biomass production. There are
large variations in the duration of lag phases following shifts from different
carbon sources. The duration of the lag phase correlates with the preshift
growth rate (Fig. 1 ): fast growth before the shift results in very long lag times.
b–d, Comparisons of lag times following filtration shifts and in diauxie
experiments (which involve no shift, but rather growth on medium containing
two sugars, with one sugar running out). b, Shift from 1.7 mM glucose to 60 mM
acetate. Here the diauxie medium contained glucose plus acetate. c, Shift from
1.7 mM glucose to 30 mM succinate. d, Shift from 1.7 mM glucose to 40 mM
pyruvate. Lag times resulting from filtration shifts and from classical diauxie
experiments are mostly comparable. In c, the presence of pyruvate in the
medium in addition to glucose adversely affected the growth rate, resulting in
a shorter lag time in the diauxie shift, consistent with our general observation
of the growth-rate dependence of lag times.