Nature - USA (2020-08-20)

a b

68 kDa

E.coli PbgA

molar mass (kDa

)

0

250

500

50

75

100

7.07.5 8.08.5

A^280

Time (min)

71 kDa

S.typhimurium PbgA

molar mass (kDa

)

0

125

250

7.07.5 8.08.5

50

75

100

Time (min)

A^280

IFD

c space

group

C2

space

group

P31

IFD

inner

membrane

cytoplasm

d phosphatidylethanolamin(partial(partial)) e

inner

leaflet

periplasmic

leaflet

monoolein

cytoplasm

periplasm

LPS

α7

PD IFD

TMD

710 Å^2

620 Å^2 1200 Å^2

e

f

TM5TM 5

D192

R215

buff er

PE PG CL PA PS

Tmelt (°C)

50

51

52

53

(^54) E.coli lipids exogenoulipids s
Extended Data Fig. 2 | See next page for caption.