5 Steps to a 5 AP Biology, 2014-2015 Edition

Molecular Genetics ❮ 135

Genetic Engineering

Restriction enzymes:enzymes that cut DNA at particular sequences, creating sticky ends.

Vector:mover of DNA from one source to another (plasmids are good vectors).

Cloning:somewhat slow process by which a desired sequence of DNA is copied numerous
times.

Gel electrophoresis:technique used to separate DNA according to size (small =faster). DNA
moves from: −to+.

Polymerase chain reaction (PCR):produces large quantities of sequence in short amount of
time.

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