Extended Data Fig. 1 | Predicted topology of OSCA1.3 with possible BIK1
phosphorylation sites and multiple alignment of loop 1 from Clade 1 OSCA
proteins. a, Topology was visualized using Protter (www.wlab.ethz.ch/protter)
version 1.0 on the basis of information from ref.^26. Blue numbers indicate
transmembrane regions. Possible BIK1 phosphorylation sites are highlighted
in red. b, Protein sequence alignment of OSCA1.1 to OSCA1.8 showing amino
acids 30 to 95. Clustal Omega alignments were visualized with Jalview 2.10.5.
Possible BIK1 phosphorylation motifs (SxxL/I) are highlighted in red. Blue
colour denotes % identity. c, Structural model for OSCA1.3. Arrows indicate the
position of S54 located in the cytosolic loop.