Extended Data Fig. 2 | Classif ication of the PARP2–HPF1–nucleosome
complex. a, Initial map generated from the entire data set, comprising
934,000 particles. The data set was further extensively classified. The regions
used for focused classifications and refinements in b–g are colour coded and
labelled. b, Cryo-EM map of nucleosome 2, refined to 2.2 Å, is shown on the left.
Fourier shell correlation (FSC) curve showing the resolution of the map
(middle). The map is coloured by local resolution. The model of the NCP (PDB
6FQ5) was refined into the cryo-EM map. The representative region showing
map quality and fit of the model is on the right. c, Angular distribution for
nucleosome 2. d, Directional FSC plot showing uniform resolution in all
directions. e, Cryo-EM map of nucleosome 1, refined to 2.8 Å, is shown on the
left. Fourier shell correlation (FSC) curve showing the resolution of the map
(middle). The map is coloured by local resolution. The model of the NCP (PDB
6FQ5) was refined into the cryo-EM map. The representative region showing
map quality and fit of the model is on the right. f, Focused classification and
refinements with a focus on the connection of the PARP2 WGR domains with
nucleosome 2 (see b). Cryo-EM map of this region was refined to 4.1 Å (left). FSC
curve showing the resolution of the map is in the middle. The model of the
PARP2 WGR domains bound to DNA (PDB 6F5B) was refined into the cryo-EM
map. The representative region showing map quality and fit of the model is on
the right. g, Focused classification and refinements with focus on the
connection of the PARP2 WGR domains with nucleosome 1 (e). Cryo-EM map of
this region was refined to 5.7 Å (left). FSC curve showing the resolution of the
map is in the middle. The model of the PARP2 WGR domains bound to DNA (PDB
6F5B) was refined into the cryo-EM map. The representative region showing
map quality and fit of the model is on the right.