Article
- Källberg, M. et al. Template-based protein structure modeling using the RaptorX web
server. Nat. Protoc. 7 , 1511–1522 (2012). - Bomati, E. K. & Noel, J. P. Structural and kinetic basis for substrate selectivity in Populus
tremuloides sinapyl alcohol dehydrogenase. Plant Cell 17 , 1598–1611 (2005). - Weigel, D. & Glazebrook, J. Transformation of agrobacterium using the freeze-thaw
method. Cold Spring Harb. Protoc. 2006 , pdb.prot4666–pdb.prot4666 (2006). - Kushnirov, V. V. Rapid and reliable protein extraction from yeast. Yeast 16 , 857–860
(2000).
Acknowledgements We thank A. Cravens for the yeast multiplex CRISPR–Cas9/sgRNA
plasmids (pCS3410, 3411, 3414 and 3700-3703); T. Lozanoski (Jarosz laboratory) for a plasmid
containing yeast codon-optimized mVenus; J. Payne for assistance with the generation of DFT
energy-optimized ligand structures for docking studies; P. Dykstra for assistance with
fluorescence microscopy and the Stanford Cell Sciences Imaging Facility for access to
microscopy equipment and training; R. Nett for suggestions regarding de novo transcriptome
assembly using Trinity; W. Cody and the Sattely laboratory for providing N. benthamiana
plants, Agrobacterium strains, a pEAQ-HT binary vector backbone plasmid, and suggestions
for tobacco agroinfiltration experiments; D. Endy and J. Payne for discussions and feedback in
the preparation of this manuscript. This work was supported by the National Institutes of
Health and the Natural Sciences and Engineering Research Council of Canada (doctoral
postgraduate scholarship to P.S.).Author contributions P.S. and C.D.S. conceived of the project, designed the experiments,
analysed the results, and wrote the manuscript. P.S. performed the experiments.
Competing interests P.S. and C.D.S. are inventors on a pending patent application. C.D.S. is a
founder and CEO of Antheia, Inc.Additional information
Supplementary information is available for this paper at https://doi.org/10.1038/s41586-020-
2650-9.
Correspondence and requests for materials should be addressed to C.D.S.
Peer review information Nature thanks Jose Avalos, Oliver Kayser and the other anonymous
reviewer(s) for their contribution to the peer review of this work. Peer reviewer reports are
available.
Reprints and permissions information is available at http://www.nature.com/reprints.