Plant Biotechnology and Genetics: Principles, Techniques and Applications

Chromatin, 88, 136–140, 144–146
Cis-acting element, 144, 164, 186
Cleistogamy, Cleistogamous flowers, 35–36
Codons, 76, 145–152, 169–170, 178–179, 220
Collins, Glenn, 130–131
Complementary DNA,seecDNA
Conner, Tony, 155–156
Cotransformation, 235–236, 259
Cre-loxrecombination system, 175–176,
360–362
Cytokinin, 100, 105, 114–116, 120, 125, 166,
230, 236

Delmer, Deborah, 108–110
Development of
Flowers, 98–101
Fruits, 88–89
Leaves, 95–98
Roots, 94–95
Dhillon, Taniya, 366–367
Disease-resistance 57, 64–5, 67, 202–205
DNA blot analysis,seeSouthern blot analysis
Doubled haploids, 75, 81, 123, 131

Electroporation, 162, 262, 267
ELISA, 277, 279–280, 288
Embryogenesis, 85–95
Enabling technologies, 326, 327,
332, 334–339
Enhancers, 143, 144, 180,
Enhancer trap, 225–226
Environmental impact quotient, 9–14
Environmental Protection Agency, US,seeEPA
Environmental Risk Assessment, 312–321
Enzyme-linked immunosorbent assay,
seeELISA
EPA, 293–296, 298–300
Epistasis, 55
EPSPS, 197–199, 207
ESTs, 196
Ethylene, 101–105, 117, 133
EU, 294, 299–301, 305, 312
European Union,seeEU
Event, transgenic, 74, 77, 162, 198, 221–224,
227, 230, 255, 259, 269, 358–360,
363, 365
Exon, 146–147
Explant, 114–128, 222
Expressed sequence tags,seeESTs

FDA, 293–298
Flavr Savr tomato, 295, 345, 350, 352

FLP-FRTrecombination system, 236, 360

Fluorescencein situhybridization, 34

Food and Drug Administration, US,seeFDA

Frankenstein, 343–344

Freedom to operate,seeFTO

GA,seeGiberellic acid

Gametogenesis, 85–88

Gateway cloning, 172–181, 361

Gene, definition, 22–23

Gene gun,seeparticle bombardment

Gene silencing,seeRNAi,see also

antisense construct

Genetic linkage, 28–29, 76, 236, 363

Genome, 22–24, 32–35, 40

Genomics, 194–197

Genotype, 25–28, 51–52

GFP reporter gene, 178, 233–234, 255,

260, 268

Gibberellic acid, 91, 101–103, 116, 185

Glyphosate resistance, 7, 9, 14, 197–199, 229,

see alsoherbicide resistance

Golden Rice, 205–207, 209, 210, 212–215

Gonsalves, 211–212

Green fluorescent protein,seeGFP

GUS reporter gene, 95, 222–224, 231–232,

268, 272

Hemizygous, 277

Herbicide resistance (or tolerance), 7, 9–14,

182, 197–200

Heterologous, 224, 332, 334

Hinchee, Maud, 270–271

Histone acetyltransferase, 145

Histones, 136, 138, 145

HPT selectable marker gene, 228–229, 332

Hybrids, 26–29, 39–44, 54–55, 61–72

Hybrid varieties, 58, 72–74

Hygromycin phophotransferase,seeHPT

selectable marker gene

IAA, 104, 114, 116

IBA, 117

Indole acetic acid,seeIAA

Indole butyric acid,seeIBA

Insect resistance, 7, 9–14, 200–202

Intellectual property,seePatents

Intron, 146–147

IPT gene, 230, 236

Isopentyl transferase gene,seeIPT gene

Jasmonic acid, 102–103

372 INDEX