Medical Microbiology

interleukinrelease,orapHtransition,candeterminewhetherantigen-spe-

cificlymphocytesarepresentorwhetherpolyclonalT-cellresponses(con-

canavalin[ConA],phytohemagglutinin[PHA])orB-cellresponses(lipopoly-

saccharide[LPS],pokeweedmitogen[PMA])wereinduced.

&Mixedlymphocytereactionsareusedtomeasurealloreactivity(prolifera-
tion,cytotoxicity),mainlybetweenrecipientsanddonorsoforganorbone

ImmunologicalTestMethods 133

Fluorescence-ActivatedCellSorter(FACS)

Application

of a charge

Pfor dropleolarized fieldt – +

deflection

Buffer

solution

Cells

Prism

Red

fluorescence

Laser

c

Forward

scattered light

(cell size)

90 ° deflection

(granulation)

Green

fluorescence

15 – 25 % 20 – 30 %

Anti-CD4

b Anti-CD8

40 – 60% <1%

Two-channel analysis

of peripheral blood lymphocytes

Number of cells

Fluorescence intensity (log 10)

Anti-CD4

Anti-CD8

a

Histogram

2

All rights reserved. Usage subject to terms and conditions of license

Kayser, Medical Microbiology © 2005 Thieme

All rights reserved. Usage subject to terms and conditions of license.