Medical Microbiology

Table 1 2. 12 Eyesandears

Infection Mostimportantpathogens Laboratorydiagnosis

Trachoma Chlamydiatrachomatis,

serovarsA,B,Ba,C

Microscopicaldetectionof

inclusionsinconjunctivalcells

(Giemsastain);directimmuno-

fluorescence;cellculture;

antigendetectionusingEIA;

PCR.Serology:recombinant

immunoassayforantibodies

togenus-specificantigen

(LPSorMOMP).Microimmuno-

fluorescenceforantibodiesto

species-andvar-specificanti-

bodies.

Conjunctivitis/scleritis

Viruses Adenoviruses

Enteroviruses

Influenzaviruses

Measlesvirus

Isolationfromswab

Bacteria Neisseriaspp.

Streptococcusspp.

Staphylococcusaureus

Haemophilusspp.

Enterobacteriaceae

Pseudomonasspp.

Mycobacteriumspp.

Moraxellalacunata

Microscopyandculturefor

bacteriainconjunctivalsecre-

tionorinscrapings

Chlamydiatrachomatis

(inclusionconjunctivitis)

Seeat“trachoma”(thistable)

Treponemapallidum Serology(basicdiagnostics)

Fungi Candidaspp.

Sporothrixschenckii

Microscopyandculturefor

fungiinconjunctivalsecretion

orincornealscrapings

Helminths Onchocercavolvulus Microscopyformicrofilariaein

skinsnips(orconjunctival)

biopsy;serology

Loaloa Microscopyformicrofilariaein

diurnalblood;serology

Eyesandears 655

12

Kayser, Medical Microbiology © 2005 Thieme