Soil Biology and Ecology
32 | Unit 2.3
PreParatiOn
SHOvEl-COUNT METHOD
For the shovel-count method, very little prepa-
ration is required. Identify sample areas, try to
collect a similar soil volume at each location,
and record results.
vERMIFUGE METHOD
The vermifuge method requires more prepara-
tion. Sample rings must be obtained or made.
Other materials must be gathered. To minimize
the amount of time needed for the demonstra-
tion, sample rings can be set out the day before.
Ideally a minimum of 4 can be set out per habi-
tat. Select areas with contrasting management
regimes. Possible habitats include orchard, row
crop, fallow, and uncultivated field soils.
To begin the demonstration, gather group at
one sample ring to explain technique. Divide
class evenly among the number of sample rings
and have each “ring-team” collect their sample.
Have one person in each team do a shovel-count
at each site for comparison. Collect results and
derive an average abundance per habitat. Ob-
serve species differences and discuss results.
PrOcedUre
- Select sample area
- Place sample rings on the surface of the site
and push them several inches into the soil. - Carefully clip vegetation and removed all
litter from inside sample area. - Slowly sprinkle 4.5 liters of vermifuge into
each sample area, distributing it evenly over
the entire surface. - After all of the vermifuge solution infiltrates
the soil, wait 10 minutes, and make a second
vermifuge application (4.5 liters). - Collect all earthworms that surface inside the
sample area. - After 10 minutes elapse since infiltration
of the second vermifuge application, use a
hand spade to dig through the surface layer
of soil (~5 cm deep) and collected any more
earthworms found there.
8. Rinse earthworms in water, drain, and store
in containers inside an insulated cooler with
ice packs (unless samples are to be counted in
the field and returned to the sample area).
9. An alternate method that does not require a
sample ring can be found in the USDA Soil
Quality Test Kit Guide, which is available on
the internet (see Resources section).
PreParatiOn tiMe
For the shovel-count method, 0.5 hour is all that
is needed. For the vermifuge method, several
hours or more may needed to gather materials.
deMOnstratiOn tiMe
1.5–2 hours
discUssiOn QUestiOns
- Most earthworm species found in farmed
soils in the U.S. were not present in those
soils 400 years ago. Where do you think they
came from? - Compare your findings from different
habitats. Which habitats had the most
earthworms per sample area? Which had the
highest diversity (greatest number of species)?
Why? - Determine what ecological types of
earthworm were present in each sample
area (see Table 2, page 34). How do you
think these results were influenced by
soil management practices in those areas.
Consider factors such as amount and type
of soil disturbance, organic matter inputs,
presence of surface organic layer, etc. - How do these findings relate to agricultural
productivity and sustainability? - If you were in charge of management
decisions for the farm soils that were
sampled, would you alter any practices based
on this information? Why?
Instructor’s Demonstration 2 Outline