Caption for Fig. 4.7(cont.)
field image (a) and white on a black background in a dark field image (b). The dark colour in the larger bristles
in (a) is produced by pigment. (c) and (d) Phase contrast view of cells growing in tissue culture. Two images
extracted from a time-lapse video sequence (time between each frame is 5 min). The sequence shows the
movement of a mouse 3T3 fibrosarcoma cell and a chick heart fibroblast. Note the bright ‘phase halo’ around
the cells. (e) and (f) Differential interference contrast (DIC) image of two focal planes of the multicellular
algaVolvox. (Images (e) and (f) courtesy of Michael Davidson, Florida State University, USA.)(a)(b)(c)Fig. 4.8Examples of different preparations in the light microscope. (a) Darkfield image of rat sperm
preparation. An aliquot was collected from an experimental protocol in order to assess the amount of damage
incurred during sonication of a population of spermatozoa. Many sperm heads can be seen in the preparation,
and the fibres of the tail are starting to fray (arrowed). (b) A brightfield image of total protein staining on
a section of a fly eye cut on a microtome, and stained with Coomassie blue. (c) DIC image of a stained
Drosophilaembryo – the DIC image shows the outline of the embryo with darker regions of neuronal staining.
The DIC image of the whole embryo provides structural landmarks for placing the specific neuronal staining
in context of the anatomy.112 Microscopy