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Colonies from cDNA library on agar plate

Isolate DNA from each clone, denature and bind to nylon membranes

Add total mRNA under hybridising conditions

Wash off unbound mRNA; single species is bound to each membrane

Elute bound mRNA

Use mRNA to direct in vitro protein synthesis

Test for production of desired protein by

procedures such as immunoprecipitation/electrophoresis

Fig. 6.29General principles involved in the technique of a hybrid select translation.

228 Recombinant DNA and genetic analysis