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For reduction reactions the working electrode is normally mercury, and for oxidative

reactions carbon or a carbon composite.

• Mass spectrometer detectors: These enable the analyte to be detected and its
  structure determined simultaneously. The technical problems associated with the
  logistics of removing the bulk of the mobile phase before the sample is introduced

Wavelength (nm)

Time (min)

240

260

280

300

320

340

360

380

400

2

4

6

8

10

12

(a)

Time (min)

(b)

mAU

0

10

20

30

2 4 6 8 10 12 14

Fig. 11.6Separation by HPLC of the dihydropyridine calcium channel blocker lacidipine and its metabolites.

Column: ODS Hypercil. Eluent: methanol/acetonitrile/water (66%, 5%, 29% by volume) acidified to pH 3.5 with

1% formic acid. Flow rate: 1 cm^3 min^1. Column temperature: 40oC. (a) As recorded by a diode array detector

and (b) by an ultraviolet detector. (Reproduced by permission of GlaxoSmithKline, UK.)

450 Chromatographic techniques