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and the term UPLC are both registered to Waters. The system operates up to 10 times

faster than conventional HPLC and chromatograms are routinely complete in

less than 5 min. Whereas conventional HPLC peaks last up to 10 s, UPLC peaks

may last 1 s so detectors have to respond ultra-fast to define the peak. The technol-

ogy is now widely used in drug discovery and metabolite identification by the

pharmaceutical industry.

1.7 μm

2.5 μm

3.5 μm

5 μm

AU

0.00

0.00

0.10

0.20

AU

0.00

0.00

0.10

0.20

AU

0.00

0.00

0.10

0.20

AU

0.00

0.00

0.10

0.20

2.00 4.00 6.00 8.00 10.00

0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00

0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00

0.20 0.40 0.60 0.80 1.00 1.10

Time (min)

Time (min)

Time (min)

Time (min)

Fig 11.7Chromatograms showing the influence of particle size on the resolution and elution time of four

analytes in an HPLC system demonstrating the time advantages of the pressure-tolerant sub-2mm ACQUITY

UPLC system. (Reproduced by permission of Waters Corporation, UK.)

452 Chromatographic techniques