Methods in Molecular Biology • 16 Enzymes of Molecular Biology

176 Pingoud, Alves, and Geiger

Table 8

Restriction Enzymes Recognizing Palindromic

Tetra- and Hexanucleotide Sequences a

oo~

A~r-~n T

A o'Vr'~r'l T

A, ,, ,v,~ T

A GC~vr~ T

A OC~o~'T

CO'Veto O

c,-,-T-," ,, o

Cmc~TmG

Cor,~'G

G~omm C

G n~'r',-~ C

Goo~m C

G oo~C

T~OoA

TO~E]A

TOO~OA

T OOOI:~vA

EcoR]

Mbol 4

M(3~II Hpdl Morn H/nPI "~ HOe411 C1o~ 1+aql "<" MSe4

Aid A¢¢114' D~I Raol

Hhol ~

NIoIU

Bb~l "t

BIll 4 NsI~II

A¢~I +

AoUI

H+ndm Aflln Cfr 101 ~ AffiU ~ ~dl Bell "I

Ndd

E¢113(il I E¢ORV

NSI:W'N Hodl

NCOI Xrnal 4 Avrll [¢o5~ Soil

S~l Os~l + Avel + Oral ~ + E~I +

S~I S~I +S~;I ,$o¢1

mspS.

So¢11 "+' P~zl

Cfr t01 Ball'Ill' Nhel BarnHI )01oll + + Bonl ~ 81p 12a Aap71.~ 8o~l-- Is.,

Norl AC)d + AC ct A¢ cl

Noel NIoN NIoN Eh~l NIoN NIoN Hincll 4

Clol + AH4 +

Nofl +:

Av~l +

Pldl

/~oLI

Bcnll ~ S0¢1" ~H "~ Bbd /koal KI~ll

Sd "14 HgiA j ~ NIIt0H I Hodl Bardl Sold + + H~[AI Sd~ + +

B~HI' A¢cnl 4 i ~l)d 8cll Foel 4-

NtpV"

41n¢11 HDol +

SnOBI + Nr~J1-4 Flml ~+ Boll + Drol +

B~AI+

"The column on the left defines the length of the recognition sequence and the phos-
phodiester bond cleaved and gives the first and last nucleotides for hexanucleotide sequences.
The row on top gives the central tetranucleotide of all recognition sequences. Listed in the
table are commercially available restriction enzymes. For sequences that are recognized by
different isoschizomers (see Table 5) a + marks the name of the enzyme sold by most manu-