226 Maunders
The temperature of reaction must be optimized for the specific appli-
cation, conditions ranging from 37°C for 30 min to 4°C overnight.
After this time, the reaction should be terminated by boiling for 2 min.
- Summary
DNA and RNA ligases are widely occurring, readily available, and
relatively inexpensive enzymes that are an essential part of the molecular
biologist's armory, both for the analysis and the manipulation of nucleic
acids. The major drawback of these enzymes lies in their relative lack
of substrate specificity as far as nucleotide sequence is involved. This
factor necessitates careful experimental design in order to obviate the
production of spurious or misinterpreted results.
References
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