260 Sharp and Slater
- Precipitate the DNA with 2 vol of ethanol at 0°C. Recover the DNA by
centrifugation at 12,000g for 10 min at 0°C in a microfuge. Remove the
supernatant, and wash the pellet with 70% ethanol. Redissolve the DNA
in 20 ~tL of the appropriate buffer (i.e., 1X restriction enzyme buffer if
further restrictions are planned).
References - Sung, S. C. and Laskowski, M., St. (1962) A nuclease from mung bean sprouts.
J. Biol. Chem. 237, 506-511. - Ardelt, W. and Laskowski, M., Sr. (1971) Mung bean nuclease I. IV. An
improved method of preparation. Biochem. Biophys. Res. Commun. 44,
1205-1211. - Johnson, P. H. and Laskowski, M., Sr. (1970) Mung bean nuclease I. I. Resis-
tance of double-stranded deoxyribonucleic acid and susceptibility of regions
rich in adenosine and thymidine to enzymatic hydrolysis. J. Biol. Chem. 245,
891-898. - Kroeker, W. D., Kowalski, D., and Laskowski, M., Sr. (1976) Mung bean
nuclease I. Terminally directed hydrolysis of native DNA. Biochemistry 15,
4463 AA.67. - Kroeker, W. D. and Kowalski, D. (1978) Gene-sized pieces produced by diges-
tion of linear duplex DNA with mung bean nuclease. Biochemistry 17, 3236-3243. - Mikulski, A. J. and Laskowski, M., Sr. (1970) Mung bean nuclease I. III. Pu-
rification procedure and (3')-c0-monophosphatase activity. J. Biol. Chem. 245,
5026-5031. - Johnson, P. H. and Laskowski, M., Sr. (1968) Sugar unspecific mung bean
nuclease I. J. Biol. Chem. 243, 3421-3424. - Martin, S. A., Ullrich, R. C., and Meyer, W. L. (1986) A comparative study of
nucleases exhibiting preference for single-stranded nucleic acid. Biochem.
Biophys. Acta. 867, 76-80. - Kowalski, D., Kroeker, W. D., and Laskowski, M., Sr. (1976) Mung bean
nuclease I. Physical, chemical and catalytic properties. Biochemistry 15,
4457-4463. - Ghangas, G. S. and Wu, R. (1975) Specific hydrolysis of the cohesive ends of
bacteriophage ~,DNA by three single strand-specific nucleases. J. Biol. Chem.
250, 4601-4606. - Jones, M. C. and Boffey, S. A. (1984) Deoxyribonuclease activities of wheat
seedlings. FEBS Lett. 174, 215-218. - Wang, J. C. (1974) Interactions between twisted DNAs and enzymes: The
effects of superhelical turns. J. Mol. Biol. 87, 797-816. - Richardson, C. C., Lehmann, I. R., and Komberg, A. J. (1964) A deoxyribo-
nucleic acid phosphatase--exonuclease from Escherichia coli. J. Biol. Chem.
239, 251-258. - von Hippel, P. H. and Felsenfeld, G. (1964) Micrococcal nuclease as a probe
of DNA conformation. Biochemistry 3, 27-39.