Food Biochemistry and Food Processing (2 edition)

(Steven Felgate) #1

BLBS102-c03 BLBS102-Simpson March 21, 2012 11:56 Trim: 276mm X 219mm Printer Name: Yet to Come


3 Enzymes in Food Analysis 41

Table 3.1.Some Commercial ELISA Kits for Protein or Allergen Analysis

Protein Source Target Protein ELISA Test Kit Supplier

Milk β-Lactoglobulin, caseins RIDASCREENβ-Lactoglobulin
RIDASCREEN Casein
Milk Protein ELISA Kit
Allergens ELISA Kits
FASTKIT SLIM Milk Allergens
ELISA Systemsβ-Lactoglobulin

R-Biopharm
R-Biopharm
Crystal Chem Inc
Astori Labs
Cosmo Bio USA
ELISA Systems
Nuts Peanut (Ara h1 and Ara h2) RIDASCREEN FAST Peanut
Peanut Protein ELISA Kit
Allergens ELISA Kits
FASTKIT SLIM Peanut Allergens
Peanut Residue ELISA

R-Biopharm
Morinaga Inst.
Crystal Chem Inc
Astori Labs
Cosmo Bio USA
ELISA Systems
Eggs Ovomucoid, ovalbumin,
ovotransferrin, lysozyme

RIDASCREEN FAST Egg Protein
Egg Protein ELISA Kit
Allergens ELISA Kits
FASTKIT SLIM Egg Allergens
Enhanced Egg Residue Assay

R-Biopharm
Crystal Chem Inc
Diagnostic Automation
Astori Labs
Cosmo Bio USA
ELISA Systems
Cereals Gliadins, secalin, hordein,
prolamin, gluten

RIDASCREEN FAST Gliadin
Gliadin ELISA Kit
Wheat Protein ELISA Kit
Gluten ELISA Kits
FASTKIT SLIM Wheat Allergens
ELISA Systems Gliadin

R-Biopharm
Morinaga Inst.
Crystal Chem Inc
ELISA Technologies
Astori Labs
Cosmo Bio USA
ELISA Systems
Mustard Mustard protein RIDASCREEN FAST Senf/Mustard
Allergens ELISA Kits
Veratox for Mustard Allergen
Mustard Seed Protein Assay

R-Biopharm
Astori Labs
Neogen
ELISA Systems
Wine, juice Lupine (e.g.,γ-conglutin) RIDASCREEN FAST Lupine
Allergens ELISA Kits
ELISA Systems Lupine Residue

R-Biopharm
Astori Labs
ELISA Systems
Crustaceans Tropomyosin RIDASCREEN FAST Crustacean
Allergens ELISA Kits
Crustacean Residue ELISA

R-Biopharm
Astori Labs
ELISA Systems
Soybean Soy trypsin inhibitor Soy Protein ELISA Kit
Allergens ELISA Kits
FASTKIT SLIM Soybean Allergens

US Biologicals
Diagnostic Automation
Astori Labs
Cosmo Bio USA
ELISA Systems

Potentiometric, amperometric, electrochemical, and piezoelec-
tric detection methods have since been developed and widely
used for these analyses.
Competitive ELISA is more flexible and applicable to anal-
ysis of small as well as large molecules. In the direct form
of the assay, the analyte of interest is first incubated together
with the enzyme-labeled antibody and the mixture added to the
analyte-specific capture antibody with which they react compet-
itively and in a concentration-dependent manner for detection
(Figure 3.2). The indirect form of competitive ELISA involves

co-incubation of the bound analyte of interest with an analyte-
specific antibody. Analyte detection is then achieved by intro-
ducing a second enzyme-labeled antibody into the mix, which
binds to the analyte-specific antibody. In another form of in-
direct competitive ELISA, the analyte-specific antibody is first
bound to the surface, and the analyte or protein of interest is
made to compete with an enzyme-labeled analyte or a tracer for
binding to the antibody. In all cases, the binding is concentration
dependent and can be measured by the changes in absorbance
or some other signal.
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