Food Biochemistry and Food Processing (2 edition)

(Steven Felgate) #1

BLBS102-c32 BLBS102-Simpson March 21, 2012 14:2 Trim: 276mm X 219mm Printer Name: Yet to Come


Table 32.2.

Summary of Transgenic Approaches to Manipulate Starch Structure and Yield in Potato Tubers

Transgenic

Starch

Grain/Tuber

Target

Approach

Content/ Yield

Starch Structure

Morphology

Application

Reference

Plastidial ATP/ADP

translocator

Antisense inhibition Starch content

decreased to 20%

Less amylose

Grains size

decreased to 50%,strange tubershape


Tjaden et al. 1998

Plastidial ATP/ADP

translocator

Overexpression of

AATP

Starch content

increased by up to30%

More amylose

Grains with angular

shape

High starch with

more amylose

Tjaden et al. 1998,

Geigenbergeret al. 2001

Plastidial adenylate

kinase

Antisense inhibition Starch content and

yield increased byup to 60%

Not determined

Not determined

High starch

Regierer et al. 2003

ADPGlc pyro-

phosphorylase

Antisense inhibition

of AGPB

Starch content

decreased down to10% of WT-level

Amylose content

decreased by 40%

Smaller grains,

smaller tubers,increased tubernumber

—M

̈uller-R

̈ober et al.

1992, Lloyd et al.1999b

ADPGlc pyro-

phosphorylase

Overexpression of

glgC

Starch content

increased by up to30%

Not determined

Not determined

High starch

Stark et al. 1991

Granule bound

starch synthase

Antisense inhibition

of GBSSI

No effect

Starch is free of

amylose

No effect

Amylose free starch Kuipers et al. 1994

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