78 1 Amino Acids, Peptides, Proteins
Table 1.34.Specificity of proteolytic enzymes [based on cleavage of oxidized B chain of bovine insulin; strong cleavage:↓, weak cleavage (↓)]
No.aPhe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys AlaSerine peptidases 1↓↓2(↓)↓↓↓3↓↓↓↓(↓
)↓↓↓(↓)4↓↓↓(↓)↓↓(↓
)5↓↓6↓↓↓↓↓↓7(↓)(↓)↓↓↓↓↓↓↓8↓↓↓(↓)↓↓↓↓(↓
)↓↓Cysteine peptidases 9↓(↓
)(↓)(↓)↓(↓)↓↓↓↓10↓↓↓↓↓↓11↓(↓
)↓↓↓↓↓↓↓(↓)(↓)Metalopeptidases 12↓↓↓↓↓↓↓↓↓↓↓13↓↓↓↓↓↓↓↓14↓↓↓↓↓↓↓↓↓↓Aspartic peptidases 15(↓)(↓)(↓)(↓)↓(↓)(↓)(↓)↓(↓)↓↓16 (↓)(↓)↓↓(↓)↓↓↓17↓↓↓↓↓18↓↓↓↓↓(↓)19(↓)(↓)↓↓↓(↓
)↓↓↓↓aEnzymes.7) Endopeptidase fromAspergillus13) Endopeptidase fromBacillus19) Endopeptidase fromRhizopus1) Trypsin (bovine)oryzaesubtilischinensis2) Chymotrypsin A (bovine)8) Endopeptidase fromAspergillus14) Thermolysin(Bacillus3) Chymotrypsin B (porcine)flavusthermoproteolyticus Rokko)4) Aspergillopeptidase C9) Papain(Papaya carica)15) Pepsin A (porcine)(Aspergillus oryzae)10) Ficin III(Ficus glabrata)16) Renin (calf)5) Endopeptidase from11) Chymopapain(Charica papaya)17) Endopeptidase fromCandidaStreptomyces griseus12) Endopeptidase II fromalbicans(trypsin-like)(Aspergillus18) Endopeptidase fromMucor6) Subtilisin BPN′oryzae)miehei