Food Chemistry

1.4 Proteins 45

Fig. 1.11.Subtilisin BPN;peptidebonds hydrolyzed by trypsin (T), chymotrypsin (C), and pepsin (P)

segment from the hydrolysis of protein by
trypsin has lysine as its C-terminal amino acid.
It is attached to the carrier with p-phenylene-
diisothiocyanate through the α-andε-amino

groups. Mild acidic treatment of the carrier

under conditions of theEdmandegradation splits

the first peptide bond. TheEdmanprocedure

is then performed on the shortened peptide