Naishet al.,Science 374 , eabi7489 (2021) 12 November 2021 4of9
Fig. 2. TheArabidopsis CEN180satellite repeat library.(A) Histograms of
CEN180monomer lengths (bp), and variant distances relative to the genome-wide
consensus. Red dashed lines indicate mean values. (B) Same as for (A) but
showing widths ofCEN180higher-order repeat blocks (monomers) and the
distance between higher-order repeats (kbp). (C) Heatmap of a representative
region within centromere 2, shaded according to pairwise variants between
CEN180.(D) Circos plot showing (i)GYPSYdensity; (ii)CEN180density; (iii)
centromericATHILA“rainfall”; (iv)CEN180density grouped by decreasing
CENH3 log 2 (ChIP/input) (red, high; navy, low); (v)CEN180density grouped by
decreasing higher-order repetition (red, high; navy, low); (vi)CEN180grouped by
decreasing variant distance (red, high; navy, low); and (vii) CENH3 log 2 (ChIP/
input) (purple) across the centromeres. (E)CEN180were divided into quintiles
according to CENH3 log 2 (ChIP/input) and mean values with 95% confidence
intervals plotted. The same groups were analyzed forCEN180variant distance
(red), higher-order repetition (blue), and CG-context DNA methylation (purple).
(F) Plot of the distance between pairs of higher-order repeats (kbp) and
divergence (variants per monomer) between the higher-order repeats. (G) Plots of
CENH3 log 2 (ChIP/input) (black) across the centromeres compared withCEN180
higher-order repetition on forward (red) or reverse (blue) strands. The heatmap
beneath is shaded according to higher-order repeat density.RESEARCH | RESEARCH ARTICLE