BIOINORGANIC CHEMISTRY A Short Course Second Edition

CALCIUM-DEPENDENT MOLECULES 309

TABLE 6.7 Interhelical Angles in Selected Calmodulin Structures Calmodulin Structure (Source) Method

Angle ( ° ) A – B (I – II)

Angle ( ° ) C – D

(III – IV)

Angle ( ° ) E – F (V – VI)

Angle ( ° ) G – H

(VII – VIII)

PDB Entry (Reference Number)

holo Ca

2+

• CaM (

Homo

sapiens

)

NMR

76 ± 2

78 ± 2

70 ± 2

78 ± 2

1J7O (N - terminal, aa 1 – 76), 1J7P

(C - terminal, aa 82 – 148) ( 68 )

apo Ca

2+

• free CaM

(Xenopus laevis

)

NMR

50 ± 2

48 ± 2

38 ± 2

53 ± 2

1F70 (N - terminal, aa 1 – 76), 1F71

(C - terminal, aa 82 – 148) ( 80 )

holo Ca

2+

• CaM

(Paramecium tetraurelia

)

X - ray

91

96

75

89

1EXR ( 67 )

holo Ca

2+

• CaM (

Homo

sapiens

)

X - ray

92

93

75

85

1CLL ( 72 )

CaM - smMLCK (

Homo

sapiens

)

X - ray

91

98

81

82

1CDL ( 86a )

CaM - CaMKII (synthetic

construct)

X - ray

89

87

83

94

1CDM ( 86b )

Source

: Reference 68.