97 Thin layer chromatography

Inthin layer chromatography(TLC) a plastic or glass plate is coated with the stationary
phase, often alumina, silica, or alkylated silica. The analyte is dissolved in a quick-drying
solvent and spotted near the bottom of the plate. The edge of the plate beneath the spot or
spots is then dipped and left in a solution of the mobile phase, either an organic solvent or
aqueous solution (depending on the nature of the analyte and stationary phase). Capillary
action is then allowed to draw thesolvent frontthrough the spotted analyte, carrying
with it and in the process separating out the analyte’s components.