and concentration measurements for marine applications.
Nortek "Vector" Acoustic Doppler Velocimeter (x3)
Nortek "Aquadopp" Acoustic Doppler Profiler
Sontek "Hydra MicroADV" Acoustic Doppler Velocimeter
Sequoia Scientific LISST Portable particle analysis system
Turner Designs "Model 10AU" Chlorophyll Fluoromenter
Turner Designs "Trinity" Flouormeter with analysis modules for chlorophyll, phycoerythrin, phycocyanin,
rhodamine, and fluorescein.
Eureka Environmental Engineering "Manta 2" Datasonde with temperature, pH,
dissolved oxygen, conductivity, and turbidity sensors
C&I Equipment Water Pumper Tank Trailer - 525 gallon tank trailer with pump for filling and dispensing
seawater.
UNCW Microscopy Facility in Dobo Hall
The microscopy facility is a dedicated 2,000 ft^2 laboratory suite located in Dobo Hall expressly
designed to accommodate light and electron microscopes. The entire suite is on a separate slab to
minimize vibrations and main power lines are routed around the suite. Two 15 ft x 15 ft rooms house a
new FEI Tecnai G2 Spirit Biotwin transmission electron microscope and a Philips XL 30S FEG scanning
electron microscope. The XL30S FEG SEM is a high-resolution instrument with secondary and
backscattered electron detectors as well as an integrated EDAX non-dispersive X-ray microanalysis unit
with both analytical and mapping software. New microscopes acquired via extramural funding from
NSF’s MRI program since 2007 include a Leica SP8 laser scanning confocal microscope, an Olympus
IX81 spinning disc confocal microscope, and an Olympus SZ dissecting microscope with infinity digital
camera.
The sample preparation area contains two full-sized hoods, ample bench space, and a darkroom,
adjacent to the transmission electron microscope, also serves the suite. Support equipment for specimen
preparation include: a Polaron Critical Point Dehydration Unit, a Leica Cryocut 1800 cryostat, a Sorval
MT-1 ultramicrotome, a Sorval MT-2 ultramicrotome, a Reichert-Jung Ultracut E ultramicrotome, an LKB
7800 glass knife maker, a Campden 725M vibrotome, a Denton DV502 vacuum evaporator, a Cressington
208HR high resolution sputter coater, as well as general histological support equipment (rotary microtomes
for paraffin sections, pH meters, mixers, incubators, balances, ovens, autoclave, etc.). New (replacement)
equipment, including a Leica UC7 ultramicrotome, Leica CM 1860 cryostat, Bio Rad PDS-1000 particle
delivery system, and Leica EM CPD300 critical point dryer, were obtained through a multi-authored NSF
MRI proposal.
A separate 10' x 30' room houses the light microscopes. The room has high and low pressure air,
positive ventilation, and computer network connections. In addition to the new microscopes listed above,
vibration free tables house the light microscopic equipment which includes: an Olympus BX60 microscope
with phase, bright field, and epifluorescence; an Olympus IMT inverted microscope with phase, bright
field, epifluorescence and Hoffman modulation contrast optics; a Zeiss Axioscope polarized light
microscope; a Zeiss Stemi SV 6 polarizing dissecting scope; a Nikon dissecting scope with split prism for
recording stereo pairs; and SPOT RT and SPOT RTke digital cameras. All of these scopes are attached to
PC’s with DVD writers and network connections. Image Pro Plus image analysis software is installed on
computers linked to the microscopes as well as to a remote station where recorded images can be analyzed.
The Olympus Fluoview 1000 Confocal Microscope has three detectors, two channels spectral and one
channel with filters. It has lasers suitable for excitation at 405/458/488/515/543/633 with AOTF control.
The spectral detectors have a 400-790 nm range, a resolution of 2nm and a speed of 140 nm/sec. It is
housed on an Olympus IX81 inverted microscope with PLAN APO lens, DIC capability and motorized Z
axis travel of 10mm with 10nm resolution.
The microscopy facility has a faculty supervisor (Dr. Alison Taylor), a full-time, university funded