6B Peptides 193
Ta b l e 6 B. 1Procedure and material used for sample preparation and techniques for separation and detection of wine peptides
Sample preparationTechnique usedReferenceProcedureMaterialPeptides separationPeptides detectionProteins precipitationTCA 7%∗Yokotsuka et al. (1975)↓↓Low pressure cationic exchangeDowex 50-X2chromatographyLow pressure cationic exchangeAmberlite IR-H 120Usseglio-Tomasset andBosia (1990)chromatographyUltrafiltrationCut-off membrane 10000RP-HPLCAbsorbance at 214 nmand fluorescence ofthe OPA derivativesAcedo et al. (1994)DaUltrasphere ODS column↓↓Low pressure exclusion chromatographySephadex LH-20Proteins precipitationEthanol 95%RP-HPLCAbsorbance at 214 nmMoreno-Arribaset al. 1996, (1998a,b);Mart ́ınez-Rodr ́ıguezet al. (2002)Nova-Pak C18 column↓↓Low pressure exclusion chromatographySephadex G-10Proteins precipitationEthanol 95%RP-HPLCScanning from 190 to280 nm and fluores-cence of the OPAderivativesBartolom ́e et al. (1997)Nova-Pak C18 column↓↓Low pressure exclusion chromatographySephadex G-10Low pressure RP-chromatographyCosmosil 140 C18-OPNRP-HPLCAbsorbance at 220 nmTakayanagi andYokotsuka (1999)μBondasphere C18 column↓↓GPCToyopearl HW-40