Wine Chemistry and Biochemistry

6B Peptides 195

Ta b l e 6 B. 1

(continued)

Sample preparation

Technique used

Reference

Procedure

Material

Peptides separation

Peptides detection

Low pressure RP- chromatography

Cosmosil 140 C

18

-OPN

RP-HPLc

Yanai et al. (2003)

↓↓

Source 5 RPC ST

GPC

Toyopearl HW-40

↓

Superdex peptide HR 10/30

Ultrafiltration

Cut-off membrane 1000 Da RP-HPLC Supercosil

ABZ+Plus

MS/MS

Person et al. (2004)

Ultrafiltration

Cut-off membrane 10,000 Da

Absorbance at 280 nm

Pozo-Bay ́

on et al. (2005)

↓↓

Low-pressure exclusion chromatography

Sephadex LH-20

Low-pressure exclusion chromatogra

phy

Sephadex LH-20

RP-HPLC Nova-Pak C18column

Absorbance at 214 nm andscanning from 190 to280 nm

Alcaide-Hidalgoet al. (2008)

↓↓

Low pressure RP-chromatography

Cosmosil 140 C

18

-OPN