SCIENCEscience.org 4 FEBRUARY 2022¥VOL 375 ISSUE 6580 575
Fig. 1. Long-term culture of PrE-derived
cells.(A) Culture conditions tested in
this study. (B) Derivation of cells in
the indicated culture conditions from
blastocysts. Derived cells at passage 2
(P2) are shown. Serum+L and Serum+C3
conditions induced XEN-like colonies,
whereas +C3PD03 induced ESC colonies.
+C3 induced distinct dome-shaped
colonies. Scale bar, 100mm. (C) Colony
morphology of CHIR99021-induced
cells maintained under the indicated
conditions for at least five passages.
Scale bar, 100mm.
Fig. 2. PrESCs retain properties of PrE.(A) PCA comparison of gene expression profiles among ESCs, TSCs, PrESCs, and XENCs. (B) Comparison of pluripotent and PrE
marker gene expression among E3.5 ICM, E4.5 ICM, ESCs, PrESCs, and XENCs. (C) Comparison of gene expression profiles of PrESCs with those of XENCs and ESCs. Red
and green dots indicate pluripotency and PrE marker genes, respectively. (D) The expression of OCT4, E-cadherin, and GATA6 in PrESCs and XENCs. Scale bar, 50mm.
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