BLBS102-c20 BLBS102-Simpson March 21, 2012 13:35 Trim: 276mm X 219mm Printer Name: Yet to Come
Table 20.4.
The Conditions for the Extraction of Collagen from Different Sources and Their Yield, Type, and Molecular Compositions
Raw Materials
Pretreatment
a
Extraction
a
%Yield (Basedon Wet Basis)
Collagen
Type
Molecular
Compositions
References
Bigeye snapper skin(Priacanthus
tayenus
, T and
Priacanthusmacracanthus
,M)
0.1 M NaOH for 6 h,
followed by 10%butanol for 18 h
0.5 M acetic acid containing pepsin
from tongol tuna stomach extract(TP) or porcine (PP) for 48 h
7.74 (T-PP)8.73 (T-TP)7.06 (M-PP)7.29 (M-TP)
Type I
(α
1)
α 2
2
Benjakul et al.
(2010)
Largefin longbarbel
catfish skin
(Mystus
macropterus
)
0.1 M NaOH containing
0.5% nonionicdetergent for 24 h,followed by 15% (v/v)butanol for 24 h and3% H
O 2
2
for 24 h
0.5 M acetic acid for 24 h and
re-extract with 0.5 M acetic acid for12 h (ASC)
0.5 M acetic acid containing porcine
pepsin for 30 h (PSC)
16.8
b
(ASC)
28.0
b
(PSC)
Type I
α
1 α
2 α
3
Zhang et al. (2009)
Seaweed pipefish(Syngnathus
schlegeli
)
0.1 M NaOH for 36 h
0.5 M acetic acid for 3 days and
re-extract with the same solution for3 days (ASC)
0.5 M acetic acid containing porcine
pepsin for 48 h (PSC)
5.5
b
(ASC)
33.2
b
(PSC)
Type I
(α
1)
α 2
2- and
α
1 α
2 α
3for
ASC and PSC,respectively
Khan et al. (2009)
Ocellate puffer fish
skin
(Takifugu rubripes
)
0.1 N NaOH for 3 days,
followed by 10%butanol for 2 days
0.5 M acetic acid for 3 days (ASC)0.5 M acetic acid containing porcine
pepsin for 48 h (PSC)
10.7
b
(ASC)
44.7
b
(PSC)
NM
d
(α
1)
α 2
2
Nagai et al. (2002a)
Bigeye snapper skin(P. tayenus
)
0.1 M NaOH for 6 h,
followed by 10% (v/v)butanol for 18 h
0.5 M acetic acid for 24 h and
re-extract with the same solution for24 h (ASC)
10.9 (ASC)
Type I
At least two
differentα
-chains
Kittiphattanabawon
et al. (2005)
Deep-sea redfish
(Sebastesmentella
)
1.0 M NaCl for 24 h
0.5 M acetic acid for 24 h and
re-extract with 0.5 M acetic acid for24 h (ASC)
0.5 M acetic acid containing porcine
pepsin for 48 h (PSC)
47.5 (ASC)92.2 (PSC)
Type I
At least two
differentα
-chains
Wang et al. (2007)
Brownstripe red
snapper (
Lutjanus
vitta
)
0.1 M NaOH for 24 h,
followed by 10% (v/v)butanol for 24 h
0.5 M acetic acid for 24 h and
re-extract with the same solution for16 h (ASC)
0.5 M acetic acid containing porcine
pepsin for 48 h (PSC)
9.0 (ASC)4.7 (PSC)
Type I
At least two
differentα
-chains
Jongjareonrak et al.
(2005)
Skate skin(Raja kenojei
)
0.1 M NaOH for 24 h
0.5 M acetic acid (ASC)0.5 M acetic acid containing porcine
pepsin for 24 h (PSC)
8.9 (NM
c)
Type I
(major)and V(minor)
(α
1(I))
α 2
2(I) with
cross-link ofα
2-chain and
(α
1(V))
α 2
2(V)
Hwang et al. (2007)
374