Organic Chemistry

Section 27.16 Laboratory Synthesis of DNA Strands 063

Tutorial:

Oligonucleotide synthesis

with phosphoramidites

O

O

O

N

base

O

O

O

O

base

Pr

Pr

P

C

solid support

O

O

O

HO

N

base

O

O

O

O

base

Pr

Pr

P

C

solid support

O

O

O

HO

N

base

O

O

O

O

base

Pr

Pr

P

C

O

O

N

O

O

O

base Pr

P

C

solid support

O

O

O

O

N

base

O

O

O

O

base

Pr

Pr

P

C

O

O

N

O

O

O

base Pr

P

C

solid support

H+

1. new monomer
   H+––tetrazole


2. I 2


3. H+

chain extension

O

a phosphate

DMTr