peak height ¥retention time. Integrated peak areas are directly proportional to
the amount of analyte chromatographed when working within the linear range
of the detector, and are the most reliable. They can be measured by computing-
integrators, by triangulation (1/2-base ¥height of the triangle that approximates
to the Gaussian peak profile) or by cutting out and weighing peaks drawn by a
chart recorder. Detector responses for an analyte are established by the prepara-
tion of a calibration graphusing chromatographed standards, with or without
the addition of an internal standard, by standard addition or by internal
normalization. These calibration procedures are described in Topics A5 and B4.130 Section D – Separation techniques