Instant Notes: Analytical Chemistry

Fig. 1. Flow chart for method validation.

A3 – Analytical techniques and methods 9

Step 1 Performance characteristics of detector

for single analyte calibration standards

Step 2 Process repeated for mixed analyte

calibration standards

Step 6 Field trials in routine laboratory with

more junior personnel to test ruggedness

Step 5 Analysis of 'spiked' simulated sample

matrix. i.e. matrix with added known

amounts of analyte(s), to test recoveries

Step 3 Process repeated for analyte calibration

standards with possible interfering

substances and for reagent blanks

Step 4 Process repeated for analyte calibration

standards with anticipated matrix

components to evaluate matrix

interference