Quick Review Cards for Medical Laboratory Science

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Haemophilus Clinical Microbiology Review 194


ORGANISM PATHOGENICITY GRAM STAIN CULTURE OTHER

H. influenzae

H. influenzaebiotype
aegyptius& H. aegyptius

H. parainfluenzae,
H. haemolyticus, &
H. parahaemolyticus

H. ducreyi

Should be serotyped.

Characteristics are
similar to H. influenzae.
Difficult to differentiate.

Can ID by PCR.

Normal flora of upper respi-
ratory tract. Causes sinusitis,
otitis media, pneumonia,
bronchitis, often in elderly or
compromised. Type b com-
mon cause of pneumonia &
meningitis in children where
Hib vaccine not available.

Both cause conjunctivitis
(pink eye). H. influenzaebio-
type aegyptiusalso causes
Brazilian purpuric fever.

Normal flora of upper respi-
ratory tract. Low incidence of
pathogenicity.

Never normal flora.
Causes chancroid (sexually
transmitted disease).

Small, pleomorphic
GNCB to long fila-
ments. Capsules
may be seen.

Same as above.

Small, pleomorphic
GNCB to long fila-
ments.

Small GNCB, bipolar
staining. May resem-
ble schools of fish or
railroad tracks.

Grows on CHOC in 5%–10%
CO 2. Translucent, moist,
tannish colonies. Encapsu-
lated strains form larger &
more mucoid colonies.
Mousy or bleach-like odor.
May demonstrate satel-
litism with staph on SBA.

Same as above.

Colonies are larger, dry,
& tannish.

Difficult to culture.
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