Routine Culture Setup and
Interpretationcontinued
Clinical Microbiology Review 222
SPECIMEN MEDIA NORMAL FLORA COMMON PATHOGENS OTHER
Wound
Eye
Ear
Practices vary. Follow laboratory’s SOP.
Gram stain.
Gram stain. If pos, call.
Keratitis is emergency. Loss
of eye can occur.
Gram stain.
CNS, diphtheroids,
P. acnes. Surgical, aspi-
rate, or tissue: none
CNS, diphtheroids,
P. acnes
Normal skin flora
SBA, CHOC, MAC.
Surgical, aspirate, or
tissue: add anaero-
bic blood, KVLB,
THIO
SBA, CHOC
SBA, CHOC, MAC
S. aureus, beta-hemolytic
strep, Enterobacteriaceae,
P. aeruginosa.Surgical, aspi-
rate, or tissue: also anaerobes.
Human bite: alpha-hemolytic
strep, S. aureus, S. pyogenes,
Eikenella corrodens, anaerobes
H. influenzae, S. pneumoniae,
S. aureus, Enterobacteriaceae,
Pseudomonas
S. pneumoniae, H. influenzae,
GAS,M. catarrhalis, S. aureus,
Pseudomonas