Grading Reactions—Tube Versus Gel Immunohematology Review 438
TUBE* GEL
4+
3+
2+
1+
Mixed fieldNeg*In tube testing, hemolysis is also a pos rxn.
Solid band of agglutinated RBCs at topBand of agglutinated RBCs near top with a few staggered belowAgglutinates throughoutAgglutinates predominantly in lower half of column with some
RBCs at bottomLayer of agglutinated RBCs at top & pellet of unagglutinated RBCs
at bottomWell-defined pellet of unagglutinated RBCs at bottomOne solid agglutinateSeveral large agglutinatesMedium-sized agglutinates, clear backgroundSmall agglutinates, turbid backgroundSome agglutinated RBCs in sea of free RBCsNo agglutinates