Amplification Terminologycontinued
Molecular Diagnostics
(MDx) Review 553
TERM DEFINITION
Thermocycler
Amplicon
Melting curve analysis
Reverse transcriptase
Copy or complementary DNA (cDNA)
Ligate
DNA ligase
Instrument that rapidly changes temperatures during amplification cycle.Product of amplification.PCR amplicons are slowly heated in presence of dyes specific for dsDNA. At low temp,
signal is high. As temp ↑, DNA strands separate & signal ↓. Fluorescent signal patterns vary
depending on nucleotide sequence.Enzyme used in vitro to make DNA from RNA. Isolated from retroviruses.DNA copied from RNA by reverse transcriptase.To join together by chemical process.Enzyme that links DNA strands by joining 5’ end of one to 3’ end of another.