- Which of the following should be chosen for best yield
if one were to produce a recombinant protein in large
amounts?
(a) Laboratory flask of largest capacity.
(b) a stirred-tank bioreactor without inlets and
outlets.
(c) a continuous culture system.
(d) all of the above.
- The role of DNa ligase in the construction of a
recombinant DNa molecule is
(a) formation of phosphodiester bond between two
DNa fragments
(b) formation of hydrogen bonds between sticky ends
of DNa fragments
(c) ligation of all purine and pyrimidine bases
(d) none of the above.
- Which of the following sequences is recognized by
restriction enzyme Bam H I?
(a) (b)
(c) (d)
- Which of the following is not a cloning vector?
(a) Cosmid (b) pbr 322
(c) Sal I (d) Phagemid
- The correct sequence of making a cell competent is
(a) treatment with divalent cations → incubation of
cells with recombinant DNa on ice → heat shock
(42°C) → placing them back on ice.
(b) heat shock (42°C) → incubation of cells with
recombinant DNa on ice → treatment with
divalent cations → placing them back on ice.
(c) treatment with divalent cations → placing cells
on ice → incubation of cells with recombinant
DNa on ice → heat shock (42°C).
(d) incubation of cells with recombinant DNa on ice
→ heat shock (42°C) → treatment with divalent
cations → placing them back on ice.
- Identify a, b, C and D in the given figure of E. coli cloning
vector pbr 322 and select the correct option.
A B C D
(a) Hin d I Eco r I ampr ori
(b) Hin d I Bam H I kanr ampr
(c) Bam H I Pst I ori ampr
(d) Eco r I Bam H I ampr ori
- If a person obtains transformants by inserting a
recombinant DNa within the coding sequence of enzyme
b-galactosidase, he will separate out recombinants
from non-recombinants by which of the following
observations?
(a) Non-recombinant colonies do not produce any
colour whereas recombinants give blue coloured
colonies.
(b) recombinant colonies do not produce any colour
whereas non-recombinants give blue coloured
colonies.
(c) recombinants and non-recombinants both
produce blue coloured colonies.
(d) No colonies are formed due to insertional inacti-
vation.
