Article
A Genome-wide CRISPR Screen in Toxoplasma
Identifies Essential Apicomplexan Genes
Graphical Abstract
Highlights
d CRISPR enables the first genome-wide loss-of-function
screen inT. gondii
d The screen measures each gene’s impact during infection of
human fibroblasts
d Functional assays validate the essentiality of formerly
uncharacterized proteins
d An invasion factor found in all apicomplexans is essential in
malarial parasites
Authors
Saima M. Sidik, Diego Huet,
SureshM. Ganesan, ...,Vern B. Carruthers,
Jacquin C. Niles, Sebastian Lourido
Correspondence
[email protected]
In Brief
The first genome-wide genetic screen of
an apicomplexan parasite identifies an
invasion factor essential for the fitness of
all parasites in this phylum, including
those causing malaria and toxoplasmosis.
Sidik et al., 2016, Cell 167 , 1423–1435
September 8, 2016ª2016 Elsevier Inc.
http://dx.doi.org/10.1016/j.cell.2016.08.019