Cell - 8 September 2016

(Amelia) #1

Supplemental Figures


Figure S1. Resolution and Model Statistics for the pED208 Pilus Structure, Related toFigure 1
(A and B) (A) Resolution of pED208, as derived from Fourier Shell Correlation (FCS) calculation between the stereochemically refined atomic model andthe map.
This yields an FSC = 0.4 at a resolution of 3.6 A ̊. Since the traditional FSC between two half-maps is not a measure of resolution, but rather of self-consistency
(Egelman, 2014), the model:map FSC provides a better measure of actual resolution. We show, however, in (B) an FSC between two independent half-maps,
which yields a resolution of 3.6 A ̊at FSC = 0.143, consistent with the model:map FSC in (A). In addition, and more importantly, the visual appearance of the map is
also consistent with a resolution of 3.6 A ̊.
(C) Model statistics from MolProbity.

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