Cell - 8 September 2016

Continued

REAGENT or RESOURCE SOURCE IDENTIFIER

Chemicals, Peptides, and Recombinant Proteins
C13.G3 This paper KX462845

426c-degly3.core-Ferritin This paper KX462844
426c-degly3.core-AviHis This paper KX518319
D426c-degly3.core-AviHis This paper KX518320

426c-degly2.core monomer This paper KX518321
426c-degly1.core monomer This paper KX518322
426c-WT.core monomer This paper KX518323

426c-WT.SOSIP This paper KX462847
eOD-GT8 60-mer This paper; made by VRC/NIAID based on
Jardine et al., 2016

KX527857

eOD-GT8-AviHis This paper; made by VRC/NIAID based on
Jardine et al., 2016

KX527855

DeOD-GT8-AviHis This paper KX527856
eOD-GT6 60-mer This paper; made by VRC/NIAID based on
Jardine et al., 2016

KX527854

eOD-GT6-AviHis This paper; made by VRC/NIAID based on
Jardine et al., 2016

KX527852

DeOD-GT6-AviHis This paper KX527854
BG505.SOSIP.T332N Made by VRC/NIAID based onSanders
et al., 2013

N/A

45-01dG5 gp120 VRC/NIAID N/A

Poly I:C Invivogen Cat# tlrl-pic-5
PE-Cy5 anti-B220 eBioscience Cat#15-0452-83
PE anti-IgM (eBioscience 12-5790-83) eBioscience Cat#12-5790-83

FITC anti-IgD PharMingen Cat# 553439
SuperScript III Reverse Transcriptase ThermoFisher Scientific Cat# 18080044
HotStarTaq Plus DNA Polymerase QIAGEN Cat# 203607

Galanthus nivalis(GNA)-lectin gel EY Laboratories Cat# A-7401-2
Ni Sepharose excel GE Healthcare Cat# 17-3712-03
Protein G Sepharose 4 Fast Flow GE Healthcare Cat# 17-0618-05

SureBlue TMB Microwell Peroxidase
Substrate

Kirkegaard & Perry Laboratories Cat# 52-00-00

Extravidin-PE Sigma Cat# E4011
Streptavidin-APC ThermoFisher Scientific Cat# SA1005

Critical Commercial Assays
Bio-Layer Interferometry Antigenicity
Analysis

ForteBio Octet HTX System

EasySep Mouse Pan-B Cell Isolation Kit StemCell Technologies Cat# 19844

Deposited Data
Paired VH:VL sequencing data This paper SRA: PRJNA327421
http://www.ncbi.nlm.nih.gov/bioproject/
PRJNA327421
HTGTS-rep-seq data This paper SRA: SRP077660
http://www.ncbi.nlm.nih.gov/sra/
SRP077660

Experimental Models: Cell Lines

Mouse: ES clone for VH1-2 mouse model This paper N/A
Mouse: ES clone for VH1-2/LC mouse
model

This paper N/A

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Cell 166 , 1471–1484.e1–e8, September 8, 2016 e3