Cell - 8 September 2016

Figure S2. Immunization of the VH1-2 Mouse Model, Related toFigure 2
(A) Gating strategy for single cell sorting of eOD-GT8-specific memory B cells in naive and eOD-GT8 60-mer-immunized mice. Splenocytes from a naive mouse
were used as a control to set up gating for the analysis of splenocytes from immunized mice.
(B) Summary of binding affinity of the cloned antibodies from eOD-GT8 60-mer immunized VH1-2 mice compared to VRC01 and its germline revertant. (C) Gene
frequency data for naive and 30mg eOD-GT8 60-mer immunized VH1-2 mice. Data were subdivided by heavy chain isotype prior to analysis.