Cell - 8 September 2016

Figure S5.gdT Cell Deletion Results in MarkedabT Cell Expansion and Activation in a Slowly Progressive Model of PDA, Related toFigures 4
and 5
(A and B) Cohorts of KC;Tcrd+/+and KC;Tcrd–/–mice were sacrificed at 3 months of life. Frozen pancreatic sections were tested for (A) CD4+and (B) CD8+T cell
infiltration by immunohistochemistry (n = 8/group).
(C–I) Pancreas draining lymph nodes from 3 month old KC;Tcrd+/+and KC;Tcrd–/–mice were harvested and tested for (C) CD4+and CD8+T cell expression of
CD44 and (D) PD-1, (E) CD8+T cell expression of ICOS and Granzyme B, (F) CD4+and CD8+T cell expression of IFN-gand (G) T-bet, and (H) CD4+T cell
expression of GATA-3 and (I) FoxP3. Representative contour plots and quantitative data are shown. Experiments were repeated 2-3 times with similar results (n =
5/group; *p < 0.05, ****p < 0.0001).